| 1. | When the aggregates reached a certain size the inner cells became lignified .
当这些聚体达到一定体积时，内部的细胞变成木质化。 |
| 2. | Upon receiving such orders , he put them in the inner cell and fastened their feet in the stocks
24禁卒领了这样的命、就把他们下在内监里、两脚上了木狗。 |
| 3. | Es cells are the cells derived from the inner cells mass ( icm ) . they are capable of differentiating in vitro into multiple cells types under the stimulation of right signals
Es细胞来源于着床前囊胚期内细胞团( innercellmass , icm ) ，具有发育全能性。 |
| 4. | After five days of growing in culture dishes , six eggs had developed into what appeared to be blastocysts , but none clearly contained the so - called inner cell mass that yields stem cells
经过五天的培养，其中有六颗卵子发育成形似囊胚的东西，只不过没有任何一个含有可产生干细胞的所谓内细胞群。 |
| 5. | Inner cell mass ( icm ) of the blastocyst stage embryo and primordial germ cells ( pgcs ) of fetus are the mostly used materials for derivation of pluripotent stem cells
早期囊胚的内细胞团( innercellmass ， icm )和原始生殖细胞( primordialgermcells ， pgcs )是获得多能干细胞( pluripotentstemcell )的两种主要的材料来源。 |
| 6. | Exoscopic describing the type of development of a plant embryo in which the apex of the sporophyte develops from the outer cell formed by the first division of the zygote , the inner cell giving rise to the foot
外向的：指一种植物胚胎的发育类型，其孢子体的顶端由受精卵的第一次分裂的外层细胞发育而来，内层细胞形成根。 |
| 7. | Purpose embryonic stem cells ( escs ) which are from the inner cell mass ( icm ) of preimplantation blastula are pluripotent . neurons are considered as terminally differentiated cells that can not divide nor proliferate after injury
目的胚胎干细胞( embryonicstemcells ， escs )来源于着床前胚胎的囊胚期内细胞团( innercellmass ， icm ) ，具有发育全能性。 |
| 8. | After 4 days of growth , the intact inner cell mass ( icm ) were separated with 0 . 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum , 0 . 1mmol / l nonessential amino acids , 0 . 1mol / l 2 - mercaptoethanol , 2mmol / l glutamine , 100 units / ml of streptomycin and 100 units / ml of penicillin . after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected
( 2 )小鼠囊胚或内细胞的培养和es细胞的分离培养了156个不同品系的小鼠囊胚，经过3 ? 4天培养后，将增殖出来内细胞团用机械法结合胰酶- edta处理，离散后培养于mef饲养层上， 4 6天后有5例出现了es细胞样集落。 |
| 9. | We used the icr and kunming mice and got the embryos of 3 . 5dpc by means of superovulation , then cultured the embryos on the feeder layer derived from icr or kunming mice , dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time . in this period , we analized the effects of feeder layer , trypsin and embryos isolated from uteri of different varietal mice on the es cell lines
以icr小鼠和昆明小鼠为实验对象，采用超数排卵的方法获得小鼠胚胎，培养在用丝裂霉素c处理过的icr小鼠和昆明小鼠mef细胞饲养层上，选取适当时间离散增殖的icm和类es细胞集落，分析了在小鼠es细胞建系过程中，饲养层细胞、消化液及不同品系来源的胚胎的影响。 |
| 10. | Embryonic stem ( es ) cells were derived from the inner cell mass ( icm ) of preimplantation embryos , and embryonic germ ( eg ) cells from primordial germ cells ( pgc ) . both es and eg cells were cultured to determine the factors affecting on isolation , cloning and passage of pluripotent stem cells from icr mouse embryos
试验以icr小鼠胚胎内细胞团( icm )和原始生殖细胞( pgc )为材料，采用不同培养体系分离克隆胚胎多能干细胞，探讨icr品系小鼠胚胎多能干细胞分离克隆的基本方法，以及一些因素对其分离、培养、克隆、传代等效果的影响。 |
